Journal of Osseointegration

Biological Response of Soft Tissues to Three Abutment Materials Titanium, Zirconia, and Lithium Disilicate. In Vitro Comparative Study.

Tue, 05 Mar 2024 00:00:00 +0000

Aim The implant-abutment/emergence connection, and therefore the relationship with soft and hard tissues, is an important factor that determines the long-term success of dental implants in clinical practice. The purpose of this study was to compare the biological response of murine fibroblasts L929 when exposed to three materials: Titanium, Zirconia, and Lithium Disilicate (DSL), used as implant abutment materials.

Materials and methods Samples of titanium, Zirconia, and DSL were obtained. Prior to material characterization by X-ray fluorescence and Raman spectroscopy, the samples were sterilized in a steam autoclave at a temperature of 121 °C for 30 minutes. Murine fibroblasts L929 were seeded for cell viability measurement. The metabolic activity was measured at 24 hours and 48 hours of culture using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The results were analyzed using SPSS v. 20.0.

Results At 24 hours, an increase in viability was observed, although there was no significant difference among the three studied materials (p=0.564). At 48 hours vs. 24 hours, DSL showed the highest degree of cell viability (p=0.0003), followed by Zirconia (p=0.027), and then Titanium (p=0.056). The cell viability values for titanium, Zirconia, and DSL were 116%, 132%, and 172%, respectively. There was no evidence of cytotoxicity.

Conclusion Cell viability in response to the studied implant abutment materials could anticipate the biological response, the stability of the different materials in relation to soft tissues, and their connection. Other factors such as biomechanics and bacterial adhesion should be considered when choosing a material.

Comparative effect of the extended use of acids for surface treatment of osseointegrated implants. A laboratory study

G. N. Mendes, W. M. Takeshita, B. F. Brasileiro, C. L. Trento — Tue, 05 Mar 2024 00:00:00 +0000

Aim The aim of this study is to comparatively analyze the effects of extended acid usage, up to the fifth time, on the treatment of dental implant surfaces on their apex, middle, and neck regions.

Materials and Methods Implant samples (n=10) were analyzed using a scanning electron microscope, and their captures by implant region (apex, body, and neck) were processed using ImageJ software. The generated Ra and Rq data of the samples were assessed using the Kruskal-Wallis test and the Dunn post-hoc test.

Results The treatment groups showed no statistical significance compared to the control group up to the fourth use. Therefore, extended acid usage is possible, but it is conditioned upon the number of acid reuses. However, in the 5th use, there was significant variation in the mean values of Ra and Rq in the Dunn post-hoc test.

Conclusion Based on the obtained results, it can be concluded that using the same acid for dental implant surface treatment up to four times did not alter the dental implant surface roughness property. Nonetheless, further studies are necessary, particularly because there is limited data regarding this unexplored research topic in the implantology literature.

Accuracy evaluation of two different intraoral scanners in implant prosthodontics. A comparative in vitro study

Tue, 05 Mar 2024 00:00:00 +0000

Aim To test differences in term of accuracy among two Intraoral Scanners used in implant fixed prosthodontics.
Materials and methods A reference stone model was prepared, representing a partially edentulous maxilla on area #23 and from #14 to #16, with three implant analogues and polyether-ether-ketone (PEEK) scanbody screwed on to represent the situation of a single crown on implant (SB) and a implant-supported partial prosthesis (2SB). The model was digitized with a laboratory scanner (Aadva lab scanner, GC, Tokyo, Japan) used as a reference, and with two intraoral scanners (Trios 3; 3Shape A/S; I700, Medit). Ten scans
were performed using the two different intraoral scanner. Scanning and processing time as well as the number of images were reordered for each scanner. All datasets were loaded into reverse-engineering software (Geomagic Control X 2018), where digital impressions were superimposed on the reference model to evaluate trueness in the full arch, in the SB area (#23) and in the2SB area(#14 and #16).Therefore, all the scans of the same group were superimposed onto the cast that recorded the best result of trueness whose trueness corresponded to the actual reference value for precision. Mann-Whitney U-test test was performed to analyze differences between the groups (P<0,05) (SPSS software Version 26,IBM).
Results Statistically significative differences where found between Medit i700 and TRIOS 3 regarding trueness and precision in the full arch , with Trios 3 showing better results than Medit I700. Trios 3 performed statistically better also in the 2SB area regarding precision.
No statistically significative differences were found regarding trueness and precision in the other ares.
Conclusions Trios 3 performed statistically significative better than Medit I700 in acquiring scanbody position when the full arch model was analyzed. Both the tested Intraoral scanners reordered good values in line with the previous literature.

Outcomes evaluation of a patient treated with roots immediate digital denture: Patient evaluation after full digital dentures

A. Casucci, G. Verniani, L. F. D’Arienzo , A. Keeling, M. Ferrari — Tue, 05 Mar 2024 00:00:00 +0000

The immediate dentures fabrication in the analogic manner may be an hight time-consuming and unreliable procedure. The purposes of this article were both to describe a simplified protocol based on a digital workflow used to fabricate a set of immediate dentures and to report patient functional and quality of life data. The digital intraoral scans were recorded and used for dentures design, the teeth arrangement proposed by the software was superimposed to patient frontal photo in order to simulate the aesthetic proposal. The resulted Standard Tessellation Language files were exported to a milling machine for denture fabrication. After immediate denture delivery the remaining lower canine roots were used to retain the prosthesis. In order to evaluate the different effects of the treatment on masticatory efficiency, bite force and health–related quality of life (OHRQoL) data were measured before and after treatment. The patient reported a good adaptation form the delivery, an improvement for all the aspects evaluated after prostheses roots anchorage.