Articles
Vol. 6 No. 3 (2014)
https://doi.org/10.23805/jo.2014.06.03.04

Osteoproperties of polyethylene glycol hydrogel material

Publisher's note
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.
Published: 30 October 2014
824
Views
673
Downloads
Adipose derived stem cells, Guided bone regeneration, Hydrogel, Osteogenesis, Polyethylene glycol

Authors

Diego Lops
info@ariesdue.it
Department of Prosthodontics, University of Milan, School of Dentistry, Dental Clinic, S. Paolo Hospital, Milano, Italy.
Letizia Ferroni
Department of Biomedical Sciences, University of Padua, Padua, Italy.
Chiara Gardin
Department of Biomedical Sciences, University of Padua, Padua, Italy.
Sara Ricci
Department of Neurosciences, University of Padua, Padua, Italy.
Riccardo Guazzo
Department of Neurosciences, University of Padua, Padua, Italy.
Luca Sbricoli
Department of Neurosciences, University of Padua, Padua, Italy.
Eugenio Romeo
Department of Prosthodontics, University of Milan, School of Dentistry, Dental Clinic, S. Paolo Hospital, Milano, Italy.
José L. Calvo-Guirado
Department of General Dentistry, Faculty of Medicine and Dentistry, University of Murcia, Murcia, Spain.
Eriberto Bressan
Department of Neurosciences, University of Padua, Padua, Italy.
Barbara Zavan
Department of Biomedical Sciences, University of Padua, Padua, Italy.
Aim The aim of the present study was to test the osteogenic potential of a synthetic hydrogel made of polyethylene glycol (PEG), loaded with adult mesenchymal stem cells, used as a biodegradable membrane for guided bone regeneration (GBR). Materials and methods Adult mesenchymal stem cells derived from adipose tissue (ADSCs) were isolated, characterized, and seeded on the hydrogel. After 15 days of culture, the scaffolds were analyzed with scanning electron microscopy (SEM) and real-time PCR to assess osteogenesis, and by means array CGH (Comparative Genomic Hybridization) to test their safety. Results The in vitro results confirmed that the ADSCs were able to attach to the hydrogel and differentiate towards the osteogenic phenotype. Furthermore, array CGH analysis detected no chromosomal abnormalities, confirming the safety of the 3D cultures. Conclusion The PEG hydrogel, loaded with adult mesenchymal stem cells, seems to have an osteogenic potential and therefore could be successfully used as a membrane in the treatment of bone defects.

Downloads

Download data is not yet available.

Citations

Supporting Agencies

How to Cite



Osteoproperties of polyethylene glycol hydrogel material. (2014). Journal of Osseointegration, 6(3), 61-65. https://doi.org/10.23805/jo.2014.06.03.04